Recombinant Streptavidin Protein INQUIRY

Cat.No: RP-106SA
Cat# RP-106SA
Product Name Recombinant Streptavidin Protein
Background Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin has been extensively applied in various biological fields, such as ELISA, IHC, TRFIA, quantifying PCR, isolation of single-stranded nucleotide, purification of biomolecule, and monoclonal antibody production as well. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Description Recombinant streptavidin protein is produced in E.coli. The molecular weight is approximately 16.5 kDa.
Source E.coli
Activity ≥13 U/mg recombinant streptavidin protein (Determined by Advanced Biomart according to modified Green Method)
Purity >95.0% as determined by SDS-PAGE.
PHYSICAL APPEARANCE Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from 5 mM PB, pH 6.3
Storage Recombinant streptavidin protein is shipped at ambient temperature, upon arrival store at -20°C. Avoid repeated freeze-thaw cycles. In lyophilized recombinant streptavidin protein for 3 years (-20°C to -80°C);
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