Recombinant Streptavidin Protein, 55kDa INQUIRY

Cat.No: RP-5912SP
Cat# RP-5912SP
Product Name Recombinant Streptavidin Protein
Background Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.
Description Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous streptavidin protein shows predominantly one single band in SDS PAGE. Recombinant streptavidin protein consists of 4 identical subunits, each bearing an active binding site for biotin. Recombinant streptavidin protein has a molecular weight of 55kDa.
Source Bacterium Streptomyces avidinii.
PHYSICAL APPEARANCE Sterile Filtered lyophilized powder.
Formulation Lyophilized (1mg/ml) in 50mM NaCl, pH 9.0.
Solubility Gives a clear solution at 5mg/ml in 0.1M NaCl.
Storage Recombinant streptavidin protein although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter. Please prevent freeze-thaw cycles.
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