Recombinant Staphylococcal Protein A (SPA) INQUIRY

Cat.No: RP-101PA

Cat#

RP-101PA

Product Name

Recombinant Staphylococcal Protein A (SPA)

Synonyms

Staphylococcal protein A, SPA, Immunoglobulin G-binding protein A, IgG-binding protein A

Introduction

Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins preferentially through interaction with the heavy chain. It is conjugated to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine for use in immunology and other biological research without affecting the antibody binding site. It is also immobilized onto solid supports such as agarose beads for total IgG purifying from ascitic fluid, serum, culture supernatants and cell extract or interest protein.

Description

Recombinant Staphylococcal Protein A produced in E.Coli is a non-glycosylated, Polypeptide chain having a molecular mass of 33.6 kDa. Recombinant Staphylococcal Protein A is purified by our proprietary chromatographic techniques.

Source

E.coli

Molecular Weight

33.6KDa(SDS-PAGE: 30 kDa)

Theoretical pI

5.10

A280nm(1 mg/mL)

0.221 

Purity

Greater than 95% as determined by SDS-PAGE

Formulation

Recombinant Staphylococcal Protein A was lyophilized from 5 mM PB, pH7.4

Storage Duration

Recombinant Staphylococcal Protein A is stable up to 3 years at -20 °C

Storage

Recombinant Staphylococcal Protein A is stable at 2-8 °C, but should be kept at -20 °C for long term storage. Avoid repeated freeze/thaw cycles.

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