Recombinant Rhesus Macaque Regulation upon Activation Normal T cell Express Sequence/CCL5 Protein INQUIRY

Cat.No: RP-1194RC
Cat# RP-1194RC
Product Name Recombinant Rhesus Macaque Regulation upon Activation Normal T cell Express Sequence/CCL5 Protein
Synonym CCL5, RANTES
Background  Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. It also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant Rhesus Macaque CCL5 contains 68 amino acids and it shares 97 % a.a. sequence identity with human CCL5.
Source E.coli
Molecular Characterization Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.
Endotoxin <0.1 EU per μg protein as determined by the LAL method.
Purity Greater than 98% as determined by SDS-PAGE and HPLC analyses
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, pH 6.0, 500mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20 C. Further dilutions should be made in appropriate buffered solutions.
Bioactivity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles.
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