Recombinant Rhesus IL 13 Protein INQUIRY

Cat.No: RP-1199RC
Cat# RP-1199RC
Product Name Recombinant Rhesus IL 13 Protein
Synonym NC30, ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789.
Background IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
Description IL 13 Rhesus Macaque Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 114 amino acids and having a molecular mass of 12.6kDa. The IL 13 Rhesus Macaque is purified by proprietary chromatographic techniques.
Source E.coli
AA Sequence SPSPVPRSTA LKELIEELVN ITQNQKAPLC NGSMVWSINL TAGVYCAALE SLINVSGCSA IEKTQRMLNG FCPHKVSAGQ FSSLRVRDTK IEVAQFVKDL LVHLKKLFRE GRFN
Purity >97.0% as determined by SDS-PAGE and HPLC analyses.
Bioactivity The ED50 as determined by the dose-dependent proliferation of TF-1 cells was < 1.0 ng/ml, corresponding to a specific activity of > 1×1,000,000 units/mg.
PHYSICAL APPEARANCE Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2?m filtered concentrated solution in PBS, pH 7.4 containing 5% trehalose.
Solubility It is recommended to reconstitute the lyophilized IL-13 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage Lyophilized IL-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-13 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
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