Recombinant Rat VEGF Protein(His Tag) INQUIRY

Cat.No: RP-2404R
Cat# RP-2404R
Product Name Recombinant Rat VEGF Protein(His Tag)
Synonym VEGF-A, Vascular permeability factor, VPF, VEGF.
Background Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells, kidney epithelial cells ).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.
Description VEGF Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 145 amino acids (206-325 a.a) and having a molecular mass of 16.7kDa. VEGF is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source E.coli
AA Sequence MGSSHHHHHH SSGLVPRGSH MGSHMAPTTE GEQKAHEVVK FMDVYQRSYC RPIETLVDIF QEYPDEIEYI FKPSCVPLMR CAGCCNDEAL ECVPTSESNV TMQIMRIKPH QSQHIGEMSF LQHSRCECRP KKDRTKPEKC DKPRR
Purity >90.0% as determined by SDS-PAGE.
PHYSICAL APPEARANCE Sterile filtered colorless solution.
Formulation VEGF protein solution (0.25mg/ml) containing Phosphate buffered saline (pH7.4) and 50% glycerol.
Storage Store at 4°C if entire vial will be used within 2-4 weeks.  Store, frozen at -20°C for longer periods of time.  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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