Recombinant Rat IGF1 Protein INQUIRY

Cat.No: RP-2328R
Cat# RP-2328R
Product Name Recombinant Rat IGF1 Protein
Synonym Somatomedin C, IGF-I, IGFIA, IGF1.
Background The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).
Description Recombinant Rat IGF1 Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. Recombinant Rat IGF1 Protein is purified by proprietary chromatographic techniques.
Source E.coli
Purity >98.0% as determined by SDS-PAGE.
Bioactivity The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0ng/ml, corresponding to a specific activity of >500,000units/mg.
PHYSICAL APPEARANCE Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The recombinant rat IGF1 protein was lyophilized with a 0.2µm filtered concentrated solution in 20mM PBS, pH 7.0.
Solubility It is recommended to reconstitute the lyophilized recombinant rat IGF1 protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage Lyophilized recombinant rat IGF1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant rat IGF1 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Online Inquiry