Recombinant Mouse EGF Protein, Biotin INQUIRY

Cat.No: RP-1728M
Cat# RP-1728M
Product Name Recombinant Mouse EGF Protein, Biotin
Synonym Urogastrone, URG, EGF.
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
Description EGF Mouse Recombinant, Biotin produced in E.coli is a non-glycosylated polypeptide chain containing 61 amino acids and having a total molecular mass of 7.0kDa. This version of EGF has a N terminal leader sequence hosting a biotin conjugation. There are 0.5 biotins for each EGF protein. 
Source E.coli
AA Sequence MKKIDDDKNS YPGCPSSYDG YCLNGGVCMH IESLDSYTCN CVIGYSGDRC QTRDLEWWEL R. 
Purity >95.0% as determined by analysis by SDS-PAGE.
Bioactivity The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells is 0.14-0.2ng/ml, corresponding to a specific activity of 7.1x106units/mg.
PHYSICAL APPEARANCE Sterile Filtered clear solution.
Formulation The protein (0.5mg/ml) solution contains sterile PBS.
Storage Should be stored at 4°C. Please do not freeze.
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