Novo™ Tn5 Transposase INQUIRY

Cat.No: TN5-001Z
Cat#: TN5-001Z

Product Name: Novo™ Tn5 Transposase

Size: 25U, 125U

Novo™ Tn5 Transposase Description: 

Tn5 Transposase is a member of the RNase superfamily of proteins which includes retroviral integrases. Tn5 can be found in Shewanella and E.coli. Novo™ Tn5 Transposase is a ​hyperactive ​form of Tn5 ​transposase. Tn5 Transposase can ​be used to ​randomly insert ​Tn5 transposon ​into target DNA.​ It ​recognizes ​inside end ​sequences, ​outside end ​sequences and ​mosaic end ​sequences of ​Tn5 transposon.​ Advanced Biomart offers high quality Tn5 transposase for in vitro transgenic (foreign gene integration into host cells) and library construction for second-generation sequencing.

Source: An E. coli strain containing the sequence of the Tn5.

Novo™ Tn5 Transposase Application:

• In vitro transgenic experiment.
• Library construction for second-generation sequencing.

Novo™ Tn5 Transposase Components:

 
Tn5 Transposase Component Name 25U Volume 125U Volume
Tn5 Transposase(1 U/μl) 25μL 25μL×5
5× LM Buffer 1mL 1mL×5
10×TPS Buffer 100μL 100μL×5

Unit Definition:

One unit Tn5 transposase is defined as the amount of enzyme that cleaves 1 ug DNA fragment containing recognition seqence in 1 hour at 37°C.

Storage: Store at -20°C

References:

1. Zhou, M., Bhasin, A., and Reznikoff, W. S. (1998) J. Mol. Biol. 276, 913-925.
2. Ason, B., Reznikoff, W. S. (2004) J. Mol. Biol. 335, 1213-1225.

 
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