Novo™ PNGase F INQUIRY

Cat.No: PNF-001RP
Cat#: PNF-001RP

Product Name: Novo™ PNGase F

Size: 15000U, 75000U

Description: 

Peptide:N-Glycosidase F, commonly kown as PNGase F, is an amidase of the peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase class. PNGase F works by cleaving between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins and glycopeptides.

Application: Glycoprotein analysis 

Source: E.coli

Molecular Weight: 36kD

Purity: 90% as determined by SDS-PAGE

Unit Definition: 

One unit is defined as the amount of enzyme required to remove greater than 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl. 

Activity: 1,800,000 U/mg

Supplied in: 50 mM NaCl, 20 mM Tris-HCl (pH 8.0 @ 25°C), 5 mM EDTA

Reaction Conditions: 

1. Add 1x Glycoprotein in Denaturing Buffer (containing 0.5%SDS,40mM DTT).
2. Denature glycoprotein by heating reaction at 100°C for 10 minutes.
3. Make a total reaction volume by adding 1% NP40, 1x Reaction Buffer[0.5 M Sodium Phosphate (pH 7.5 @ 25°C)] and PNGaseF.
4. Incubate reaction at 37°C for 1 hour. 

Storage: -20°C

 
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