Mouse CD14 Development ELISA Kit INQUIRY

Cat.No: ECD-134
Cat# ECD-134
Product Name Mouse CD14 Development ELISA Kit
Size 5plates, 15plates
Description The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
Intend Use Mouse CD14 Development ELISA Kit is for the quantitative determination of Mouse CD14. This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on 96-well plates.
Principle Of The Test The Mouse CD14 Development ELISA Kit is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD14 coated on a 96-well plate. Standards and samples areadded to the wells, and any CD14present binds to theimmobilized antibody. The wells are washed and a horseradish peroxidaseconjugated rabbit anti-CD14 monoclonal antibody isthen added, producing an antibody-antigen-antibody "sandwich". The wells areagain washed and TMB substrate solution is loaded, which produces color inproportion to the amount of CD14present in thesample. To end the enzyme reaction, the stop solution is added and absorbancesof the microwell are read at 450 nm.
Sensitivity The minimum detectable dose of Mouse CD14 was determined to be approximately 78.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Materials Provided Capture Ab: 0.5 mg/mL of mouse anti-mouse CD14 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab: 0.5 mg/mL rabbit anti-mouse CD14 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use
Standard:  Each vial contains 90 ng of recombinant mouse CD14. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 to -80 in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.
Storage Capture Antibody: Aliquot and store at -20 to -80 for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 to -80 and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20 to -80 for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 for up to 1 month. Avoid repeated freeze-thaw cycles.
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