Cat.No: ETRK-133
Cat# ETRK-133
Product Name Human Trk-B / NTRK2 ELISA Kit
Size 5plates, 15plates
Description TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways.
Intend Use The Human Trk-B / NTRK2 ELISA Kit is for the quantitative determination of Human Trk-B / NTRK2. This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on 96-well plates.
Principle Of The Test The Human Trk-B / NTRK2 ELISA Kit is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Trk-B /NTRK2 coated on a 96-well plate. Standards and samples are added to the wells,and any Trk-B / NTRK2 present binds to the immobilized antibody. The wells arewashed and a horseradish peroxidase conjugated mouse anti-TrkB monoclonalantibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of Trk-B / NTRK2 present in the sample. Toend the enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.
Sensitivity The minimum detectable dose of human Trk-B / NTRK2 was determined to be approximately 6.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Materials Provided Capture Ab: 0.3 mg/mL of mouse anti-TrkB monoclonal antibody. Dilute to a working concentration of 0.5 μg/mL in CBS before coating.
Detection Ab: 0.95 mg/mL mouse anti-TrkB monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use
Standard: Each vial contains 120 ng of recombinant TrkB. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 to -80 in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
Storage Capture Antibody: Aliquot and store at -20 to -80 for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 to -80 and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20 to -80 for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 for up to 1 month. Avoid repeated freeze-thaw cycles.
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