Cat.No: EFGFR-132
Cat# EFGFR-132
Product Name Human FGFR4 / CD334 ELISA Kit
Size 5plates, 15plates
Description Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor, is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution.
Intend Use The Human FGFR4 / CD334 ELISA Kit is for the quantitative determination of Human FGFR4. This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on 96-well plates.
Principle Of The Test Human FGFR4 / CD334 ELISA Kit is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for FGFR4 / CD334 coated on a 96-well plate. Standards and samples are added to the wells, and any FGFR4 / CD334 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase(HRP) conjugated rabbit anti-FGFR4 / CD334 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of FGFR4 / CD334 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Sensitivity The minimum detectable dose of Human FGFR4 / CD334 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Materials Provided Capture Ab: 0.5 mg/mL of mouse anti-FGFR4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating.
Detection Ab: 0.25 mg/mL rabbit anti-FGFR4 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard: Each vial contains 42 ng of recombinant FGFR4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 to -80 in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
Storage Capture Antibody: Aliquot and store at -20 to -80 for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 to -80 and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized Standard at -20 to -80 for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 for up to 1 month. Avoid repeated freeze-thaw cycles.
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